Des; Primary recovery
2015-10-29 04:34:44 0 举报
Primary recovery，也被称为初次回收或初级回收，是废物管理的一种策略。它涉及将废物转化为可再利用的资源，如金属、纸张、塑料等。这种方法旨在减少废物的总量，降低对环境的影响，同时节约资源。通过有效的分类和回收，我们可以将许多原本被丢弃的物品转化为有价值的资源。例如，废旧的报纸可以回收制成新的纸张，旧的铝罐可以熔化并重新制成新的产品。这种回收方法对于保护环境、节约资源和推动可持续发展具有重要意义。然而，有效的初级回收需要公众的积极参与和政府的有力支持，包括提供适当的回收设施和教育公众关于废物分类和回收的重要性。
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CELL LYSIS & HEAT TREATMENT(Protein release)
CENTRIFUGATION(Cell debris removal)
CENTRIFUGATION(Cell harvest)
FILTRATION(Pre-capture step treatment)
SEED TRAIN 1+2(inoculation and expression)
FERMENTATION(cell growth and expression)
Tris/EDTA\t Suitable scale of operation: small to large scale operation Speed of operation: 5 or 20 L Volumetric power consumption: 2 W/kg Temperature of 65 oC.\t Operation time: Mild overnight agitation Heat treatment: Tri/EDTA can be combined with high temp (~25-65 oC) Fab demonstrates high thermal stability; Many E. coli proteins and incomplete or incorrectly assembled antibody fragments are unstable at high temperatures incubation at 65oC can pre-clear some host proteins thereby achieving enrichment of product stream Reference: Weir and Bailey (1997)
Centrifugation coupled with depth filtration has become themain set of unit operations employed for the primary recovery Advantages: Easy to scale-up Quick and efficient Constant cell removal Operation: 1. Pre-dilute the homogenate feed-stream to 4% (ww/v) 2. 1500 L/h Results: 1. Yield: ~ 94% 2. Clarification: ~ 50%
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