Experiment Design (with references)
2016-03-08 16:48:51 1 举报Experiment Design (with references)
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References
Test:1. Function of enzymes2. Self-assembly properties of hydrophobin[21]
End In-vitro Expression
In-vitro:Impact of pH and electrolyte[22]
STEP I
Modification on conditions
Experiment Design
Construction
End
In-vitro Expression
In-vivo Expression
If failed
Mathematical modeling: (yield)-(rate of 3 enzymes)
STEP IV
Start
Modification considering factors influencing the expression level of exogenous protein in Pichia Pastoris
STEP III
If successful
STEP II
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