Production Work Flow
2022-03-21 10:47:52 5 举报抗体生产流程 antibody production
作者其他创作
大纲/内容
Cherry Pick
Immunization
Initicate project
Splenocyte/PBMC isolation
rabbit pick
FACS AssayQuantitationKineticsEpitope BinningAffinity Ranking
Monoclonal Antibody Discovery Flow Chart
Production
Addition Assay
LEM construction
select clone
Plasmid sup test
Ag screening
Phase III
Immunologic Assay
Phase IIb
OSLIC
Molecular Biology
small scale
purification
Sequencing
Bleed ELISA
IgG test
B cell Sorting
Initiate immunization
Phase IIa
QA: Ag binding
counterscreening
mAbs production
QA: SDS-PAGE
Client / PM
Cell biology
Phase I
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